Sialyltransferases are enzymes that transfer sialic acid to nascent oligosaccharide. Each sialyltransferase is specific for a particular sugar substrate. Sialyltransferases add sialic acid to the terminal portions of the sialylated glycolipids (gangliosides) or to the N-or O-linked sugar chains of glycoproteins. Sialyltransferases belong to glycosyltransferase family 29 (CAZY GT_29) which use a nucleotide monophosphosugar as the donor (CMP-NeuA) instead of a nucleotide diphosphosugar.
α-2,3-Sialyltransferase, Recombinant (Photobacterium damsela)
Lyophilized powder containing Tris-HCl and NaCl.
Enzyme Commission Number
One unit will catalyze the formation of 1.0 mmole of Neu-5-Ac-a-2,3-Lac-MU from CMP-Neu-5-Ac and Lac-b-O-MU per minute at 37 °C at pH 8.0.
Store the product at –20 °C. It remains active for at least 1 year when stored properly.
Reconstitute the lyophilized powder with water to ~5 mg/mL. Solutions can be stored at 2–8 °C for 1–2 months after reconstitution. They can also be aliquoted and frozen at –70 °C or –20 °C for 1 year. Multiple freeze-thaw cycles should be avoided.
α (2,3)-Sialyltransferase; Beta-galactoside alpha-2,3-sialyltransferase; Beta-galactosamide alpha-2,3-sialyltransferase; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase