In enzymology, a sulfate adenylyltransferase (EC 188.8.131.52) is an enzyme that catalyzes the chemical reaction:ATP + sulfate↔ diphosphate + adenylyl sulfate. Thus, the two substRates of this enzyme are ATP and sulfate, whereas its two products are diphosphate and adenylyl sulfate. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing nucleotide groups (nucleotidyltransferases). This enzyme participates in 3 metabolic pathways:purine metabolism, selenoamino acid metabolism, and sulfur metabolism.
ATP Sulfurylase, Recombinant (S. cerevisiae)
ATP Sulfurylase catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi.
10 mM Tris-HCl (pH 7.5), 50 mM NaCI, 0.1 mM EDTA, 0.1 mM DTT and 50% glycerol.
One unit is defined as the amount of enzyme that catalyzes the conversion of 1 µmol of APS and PPi to ATP in one minute at 30°C in a total reaction volume of 40 µl.
ATP-sulfurylase; adenosine-5'-triphosphate sulfurylase; adenosinetriphosphate sulfurylase; adenylylsulfate pyrophosphorylase; ATP sulfurylase; sulfurylase; EC 184.108.40.206; 9012-39-9; Sulfate adenylate transferase