CHEK2 is the official symbol for the human gene Checkpoint kinase 2. CHEK2 is located on the the long (q) arm of chromosome 22. CHEK2 is tumor suppressor gene that encodes the protein CHK2, a serine threonine kinase. CHK2 operates in an intricate network of proteins to elicit DNA repair, cell cycle arrest or apoptosis in response to DNA damage. Mutations to the CHEK2 gene have been linked to a wide range of cancers including breast cancer.
CHEK2, Recombinant (Human)
Kinase activity is measured as the molar amount of phosphate incorporated into the CHKtide per minute per mg protein at 30°C using a final concentration of 50 μM [32P] ATP.
buffered aqueous glycerol solution, recombinant, expressed in E. coli, > 85% (SDS-PAGE)
buffered aqueous glycerol solution
protein apparent mol wt ~88 kDa
Supplied at a concentration of approximately 0.1 mg/mL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol
Cell Cycle, organism-specific biosystem; Cell Cycle Checkpoints, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; FOXM1 transcription factor network, organism-specific biosystem; G1/S DNA Damage Checkpoints, organism-specific biosystem
ATP binding; metal ion binding; nucleotide binding; protein binding; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity; protein serine/threonine kinase activity; protein serine/threonine kinase activity
Checkpoint Kinase 2; CHEK2; CDS1; CHK2; HuCds1; LFS2; PP1425; RAD53; hCds1; Chek2; Chk2