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Chemically modified Glycerol-3-phosphate Oxidase from E. coli

Cat No.
DIA-287
Description
Recombinant oxidoreductase that catalyzes the interconversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Take advantage of the enhanced liquid stability of this enzyme. Rely on the proven diagnostic quality of this product.
Abbr
Chemically modified GPO, Recombinant (E. coli)
Alias
GPO
Source
E. coli
Applications
Use Glycerol-3-phosphate Oxidase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase and Lipoprotein Lipase.
Appearance
Green yellow amorphous lyophilizate
Activity
>10 U/mg lyophilizate (+37°C, L-α-glycerol phosphate); Specific activity (+25°C): >40 U/mg protein
Contaminants
Cholesterol oxidase: <0.001 Lactate oxidase: <0.002 Uricase: <0.05
Molecular Weight
75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G 150)
Isoelectric point
~4.2
pH Stability
6.5-8.5
Michaelis Constant
K-phosphate buffer, 0.1 mol/l; pH 7.5: 1.36 x 10-2 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 7.6: 2.90 x 10-3 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 8.1: 1.40 x 10-3 mol/l (PAP assay)
Specificity
Glycerol phosphate oxidase reacts highly specific with L-α-glycerol phosphate.
Optimum pH
8.0-8.5
Stability
At +2 to +8°C within specification range for 12 months. Store dry.
Inhibitors
Ag, Hg-salts and SDS
Synonyms
glycerol-3-phosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; GPO
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