Recombinant oxidoreductase that catalyzes the interconversion of glycerol 3-phosphate to dihydroxyacetone phosphate. Take advantage of the enhanced liquid stability of this enzyme. Rely on the proven diagnostic quality of this product.
Chemically modified GPO, Recombinant (E. coli)
Use Glycerol-3-phosphate Oxidase in diagnostic tests for the determination of triglycerides together with Glycerol Kinase and Lipoprotein Lipase.
Green yellow amorphous lyophilizate
>10 U/mg lyophilizate (+37°C, L-α-glycerol phosphate); Specific activity (+25°C): >40 U/mg protein
Cholesterol oxidase: <0.001 Lactate oxidase: <0.002 Uricase: <0.05
75 kD (SDS-PAGE); 74 kD (gel filtration, Sephadex G 150)
K-phosphate buffer, 0.1 mol/l; pH 7.5: 1.36 x 10-2 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 7.6: 2.90 x 10-3 mol/l (o-dianisidine assay) Tris buffer, 0.1 mol/l; pH 8.1: 1.40 x 10-3 mol/l (PAP assay)
Glycerol phosphate oxidase reacts highly specific with L-α-glycerol phosphate.
At +2 to +8°C within specification range for 12 months. Store dry.
Ag, Hg-salts and SDS
glycerol-3-phosphate oxidase; sn-glycerol-3-phosphate: oxygen 2-oxidoreductase; glycerol phosphate oxidase; glycerol-1-phosphate oxidase; glycerol phosphate oxidase; L-alpha-glycerophosphate oxidase; alpha-glycerophosphate oxidase; L-alpha-glycerol-3-phosphate oxidase; GPO