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Creatine Kinase BB Fraction Human, Recombinant

Cat No.
NATE-0139
Description
Brain-type creatine kinase also known as CK-BB is a creatine kinase that in humans is encoded by the CKB gene. The protein encoded by this gene, CK-BB, consists of a homodimer of two identical brain-type CK-B subunits. BB-CK is a cytoplasmic enzyme involved in cellular energy homeostasis, with certain fractions of the enzyme being bound to cell membranes, ATPases, and a variety of ATP-requiring enzymes in the cell.
Abbr
CKBB, Recombinant (Human)
Alias
CKB; CKBB; B-CK
Source
Pichia pastoris
Species
Human
Applications
Human creatine kinase BB fraction has been used to investigate the survival benefit of the late percutaneous coronary intervention in patients after acute myocardial infarction. Human creatine kinase BB fraction has also been used in a study to analyze protein oxidations and resultant loss of function.
Product Overview
> 90% (SDS-PAGE), liquid, recombinant, expressed in Pichia pastoris
Form
liquid
Purity
> 90% (SDS-PAGE)
Concentration
> 1.0 mg/mL
Unit Definition
One unit will transfer 1.0 μmole of phosphate from creatine phosphate to ADP per minute at 37°C (measured at 340 nm as one equimolar amount of NADH produced by coupled reaction).
Stability
−70°C
Buffer
Solution in 50% glycerol, Tris buffer with 10 mM Bis-Tris-HCl, 0.5mM DTT, 0.5mM EDTA
Pathway
Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Creatine metabolism, organism-specific biosystem; Creatine pathway, organism-specific biosystem; Creatine pathway, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem
Function
ATP binding; creatine kinase activity; nucleotide binding
Synonyms
CKB; creatine kinase, brain; CKBB; creatine kinase B-type; creatine kinase-B; creatine kinase B chain; B-CK; Brain-type creatine kinase; Creatine Kinase BB; CK-BB; BB-CK
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0958
EC No.EC 2.7.3.2
CAS No.9001-15-4
SourcePorcine Brain
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