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Creatine Kinase MM Isoenzyme Type-1 from Human, Recombinant

Cat No.
NATE-0820
Description
Creatine Kinase MM is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Abbr
CKMMT1, Recombinant (Human)
Alias
CKMMT1; M-CK; CKM; CKMM; CKMMITI
Source
Pichia Pastoris
Species
Human
Appearance
Sterile Filtered colourless liquid formulation.
Product Overview
CKMT1 Human Recombinant without C-terminal Lysine on both chains produced in Pichia Pastoris is a glycosylated 47kDa polypeptide chain having an identical amino acid sequence compared to the native enzyme, purified under non-denaturing conditions and reacts with polyclonal antibodies to MM Isoenzyme in ELISA. The CKMT1 is purified by proprietary chromatographic techniques.
Enzyme Commission Number
EC 2.7.3.2
Activity
537 IU/mg
Molecular Weight
47kDa
Purity
Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Stability
CKMT1 although stable at 15°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles.
Buffer
CKMT1 contains 0.01M Tris-HCl, 0.075M NaCl, 10mM beta-mercaptoethanol, 50% glycerol, 0.1% sodium azide, pH 7.2.
Synonyms
Creatine kinase M-type; EC 2.7.3.2; Creatine kinase M chain; M-CK; CKM; CKMM; CKMMITI; CKMMT1
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