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Enzymes for Research, Diagnostic and Industrial Use

Diaphorase from E. coli, Recombinant

Cat No.
DIA-320
Abbr
Diaphorase, Recombinant (E. coli)
Source
E. coli
Applications
High purity Diaphorase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.8.1.4
Activity
12 U/mg protein at pH 9.0 and 25°C.
Molecular Weight
~ 51,700
Isoelectric point
~ 6.0
Unit Definition
One Unit of diaphorase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
Optimum pH
9
Thermal stability
up to 25°C
Stability
> 2 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in 0.5 mM FAD containing 0.5 mg/ml BSA. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
dehydrolipoate dehydrogenase; diaphorase; dihydrolipoamide dehydrogenase; dihydrolipoamide:NAD+ oxidoreductase; dihydrolipoic dehydrogenase; dihydrothioctic dehydrogenase; lipoamide dehydrogenase (NADH); lipoamide oxidoreductase (NADH); lipoamide reductase; lipoamide reductase (NADH); lipoate dehydrogenase; lipoic acid dehydrogenase; lipoyl dehydrogenase; dihydrolipoyl dehydrogenase; EC 1.8.1.4; 9001-18-7; Lipoamide Dehydrogenase
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