Diaphorase, Recombinant (E. coli)
High purity Diaphorase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
In 3.2 M ammonium sulphate.
Enzyme Commission Number
12 U/mg protein at pH 9.0 and 25°C.
One Unit of diaphorase is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
up to 25°C
> 2 years at 4°C.
For assay, this enzyme should be diluted in 0.5 mM FAD containing 0.5 mg/ml BSA. Swirl to mix the enzyme suspension immediately prior to use.
dehydrolipoate dehydrogenase; diaphorase; dihydrolipoamide dehydrogenase; dihydrolipoamide:NAD+ oxidoreductase; dihydrolipoic dehydrogenase; dihydrothioctic dehydrogenase; lipoamide dehydrogenase (NADH); lipoamide oxidoreductase (NADH); lipoamide reductase; lipoamide reductase (NADH); lipoate dehydrogenase; lipoic acid dehydrogenase; lipoyl dehydrogenase; dihydrolipoyl dehydrogenase; EC 22.214.171.124; 9001-18-7; Lipoamide Dehydrogenase