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Galactose dehydrogenase/Galactose mutarotase from E. coli, Recombinant

Cat No.
NATE-1070
Description
In enzymology, a galactose 1-dehydrogenase (EC 1.1.1.48) is an enzyme that catalyzes the chemical reaction: D-galactose + NAD+ rightleftharpoons D-galactono-1,4-lactone + NADH + H+. Thus, the two substrates of this enzyme are D-galactose and NAD+, whereas its 3 products are D-galactono-1,4-lactone, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. This enzyme participates in galactose metabolism. An aldose 1-epimerase (EC 5.1.3.3) is an enzyme that catalyzes the chemical reaction:alpha-D-glucose↔ beta-D-glucose. Hence, this enzyme has one substrate, alpha-D-glucose, and one product, beta-D-glucose. This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. This enzyme participates in glycolysis and gluconeogenesis.
Abbr
Galactose dehydrogenase/Galactose mutarotase (E. coli), Recombinant
Source
E. coli
Species
E. coli
Applications
High purity recombinant Galactose dehydrogenase/Galactose mutarotase for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 1.1.1.48 and EC 5.1.3.3
Activity
Galactose dehydrogenase (GalDH); > 200 U/mL at pH 8.6 and 25°C. Mutarotase; 4.1mg/Ml
Purity
Each protein appears as a single band on SDS-gel electrophoresis and on isoelectric focusing.
Unit Definition
One Unit of galactose dehydrogenase (GalDH) is defined as the amount of enzyme required to produce one μmole of NADH from NAD+ per minute.
Optimum pH
8.6
Optimum temperature
20°C – 40°C
Stability
> 2 years at 4°C.
Storage
4°C
Preparation Instructions
For use in the measurement of lactose and D-galactose, refer to the Lactose/D-Galactose Assay Kit for details of required concentrations, aliquots and incubation times. Swirl to mix the enzyme suspension immediately prior to use.
Synonyms
mutarotase; aldose mutarotase; galactose mutarotase; galactose 1-epimerase; D-galactose 1-epimerase; aldose 1-epimerase; EC 5.1.3.3; D-galactose:NAD+ 1-oxidoreductase; D-galactose dehydrogenase; beta-galactose dehydrogenase; NAD+-dependent D-galactose dehydrogenase; galactose 1-dehydrogenase; EC 1.1.1.48; Galactose dehydrogenase
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