Aspartate transaminase (AST) or aspartate aminotransferase, also known as AspAT/ASAT/AAT or serum glutamic oxaloacetic transaminase (SGOT), is a pyridoxal phosphate (PLP)-dependent transaminase enzyme (EC 184.108.40.206). AST catalyzes the reversible transfer of an α-amino group between aspartate and glutamate and, as such, is an important enzyme in amino acid metabolism. AST is found in the liver, heart, skeletal muscle, kidneys, brain, and red blood cells. Serum AST level, serum ALT (alanine transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.
GOT, Recombinant (E. coli)
High purity Glutamate oxaloacetate transaminase (E. coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
In 3.2 M ammonium sulphate
Enzyme Commission Number
180 U/mg protein at pH 8.5 and 25°C.
One Unit of L-glutamic-oxaloacetic transaminase (GOT) is defined as the amount of enzyme required to produce one μmole of NAD+ from NADH.
up to 25°C
> 4 years at 4°C
For assay, this enzyme should be diluted in 3 µM pyridoxal phosphate containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
EC 220.127.116.11; glutamic-oxaloacetic transaminase; glutamic-aspartic transaminase; transaminase A; AAT; AspT; 2-oxoglutaRate-glutamate aminotransferase; aspartate α-ketoglutaRate transaminase; aspartate aminotransferase; aspartate-2-oxoglutaRate transaminase; aspartic acid aminotransferase; aspartic aminotransferase; aspartyl aminotransferase; AST; glutamate-oxalacetate aminotransferase; glutamate-oxalate transaminase; glutamic-aspartic aminotransferase; glutamic-oxalacetic transaminase; glutamic oxalic transaminase; GOT (enzyme); L-aspartate transaminase; L-aspartate-α-ketoglutaRate transaminase; L-aspartate-2-ketoglutaRate aminotransferase; L-aspartate-2-oxoglutaRate aminotransferase; L-aspartate-2-oxoglutaRate-transaminase; L-aspartic aminotransferase; oxaloacetate-aspartate aminotransferase; oxaloacetate transferase; aspartate:2-oxoglutaRate aminotransferase; glutamate oxaloacetate transaminase; 9000-97-9