In enzymology, a N-acylmannosamine 1-dehydrogenase (EC 184.108.40.206) is an enzyme that catalyzes the chemical reaction:N-acyl-D-mannosamine + NAD+↔ N-acyl-D-mannosaminolactone + NADH + H+. Thus, the two substrates of this enzyme are N-acyl-D-mannosamine and NAD+, whereas its 3 products are N-acyl-D-mannosaminolactone, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
N-acylmannosamine 1-dehydrogenase, Recombinant (Pseudomonas sp.)
lyophilized powder; Powder also contains bovine albumin and sucrose.
Enzyme Commission Number
> 45 units/mg protein
mol wt ~120 kDa (gel filtration)
One unit will oxidize 1.0 μmole of N-acetyl-D-mannosamine to N-acetyl-D-mannosaminolactone per minute at pH 8.2 at 37°C in the presence of NAD.
0.1 M Tris-HCl, pH 8.2: soluble 5 mg/mL
N-acylmannosamine 1-dehydrogenase; EC 220.127.116.11; N-acylmannosamine dehydrogenase; N-acetyl-D-mannosamine dehydrogenase; N-acyl-D-mannosamine dehydrogenase; N-acylmannosamine dehydrogenase; 117698-08-5