In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
3-HBDH, Native (Alcaligenes faecalis)
Useful for enzymatic determination of ketone bodies
Is an enzyme produced by microorganisms. This product shall be used for a diagnostics reagent.
Enzyme Commission Number
NADH oxidase < 0.0004%
60±5 kDa (TSK G‒3000SW); 30±5 kDa (SDS‒PAGE)
5.5-11.0 (37°C, 60 mins)
D‒3‒Hydroxybutyrate 1.6 × 10-3M
One unit is defined as the amount of enzyme which converts 1 μ mole of 3‒Hydroxybutylate to acetoacetate per minute at 37°C under the conditions specified in the assay procedure.
45°C (Tris‒HCl buffer)
Stable at 37°C and below (pH 8.5, 10 mins)
3-hydroxybutyrate dehydrogenase; 3-HBDH; NAD-β-hydroxybutyRate dehydrogenase; hydroxybutyRate oxidoreductase; β-hydroxybutyRate dehydrogenase; D-β-hydroxybutyRate dehydrogenase; D-3-hydroxybutyRate dehydrogenase; D-(–)-3-hydroxybutyRate dehydrogenase; β-hydroxybutyric acid dehydrogenase; 3-D-hydroxybutyRate dehydrogenase; β-hydroxybutyric dehydrogenase; EC 18.104.22.168; 9028-38-0