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Native Bacillus licheniformis Alkaline Protease

Cat No.
NATE-0444
Description
Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.
Abbr
MCP, Native (Bacillus licheniformis)
Alias
ingensin; macropain; prosome; MCP; proteasome
Source
Bacillus licheniformis
Applications
The enzyme has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart.1 It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA. This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.
Product Overview
Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Form
lyophilized powder
Enzyme Commission Number
EC 3.4.25.1
Activity
7.0-14.0 units/mg
CAS No.
140879-24-9
Molecular Weight
27 Kda
Unit Definition
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent).
Synonyms
ingensin; macropain; multicatalytic endopeptidase complex; prosome; multicatalytic proteinase (complex); MCP; proteasome; large multicatalytic protease; multicatalytic proteinase; proteasome organelle; alkaline protease; 26S protease; triCorn proteinase; triCorn protease; EC 3.4.25.1
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