A thermostable 4-α-glucanotransferase that elongates linear alpha-glucan chains in starch and amylose by catalyzing the transfer of one glucose unit from the non-reducing end to a new position in an acceptor, which may be triose or larger. The enzyme can be used various applications, such as for modifications of alpha-polysaccharides and is ideal for making clear size ladders of oligosaccharides larger than maltose. The enzyme was developed from metagenome DNA obtained from environmental sample from a geothermal environment.
Glucan elongation enzyme, Native (Bacterial Environmental DNA)
Bacterial Environmental DNA
The enzyme catalyzes the formation of the alpha-1,4-glucosidic linkages. The enzyme transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan.
Half unit (U) is defined as the amount of enzyme which lowers the OD at 650 nm from 1.2 to 0.7 in 30minutes.
pH optimum is around 7
The enzyme in relatively active in a rather broad temperature range with optimum around 70°C
Glucan elongation enzyme