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Native Baker's yeast (S. cerevisiae) Inorganic Pyrophosphatase

Cat No.
NATE-0354
Description
Pyrophosphatase (or inorganic pyrophosphatase) is an enzyme (EC 3.6.1.1) that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis,as well as other biochemical transformations.
Abbr
Inorganic pyrophosphatase, Native (Baker's Yeast (S. cerevisiae))
Source
Baker's yeast (S. cerevisiae)
Applications
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi. It plays an important role in protein, RNA, and DNA synthesis.
Product Overview
This ubiquitous enzyme serves to drive metabolic reactions that produce pyrophosphate, since these reactions typically have equilibrium constants near unity. The catalytic mechanism has been described in the literature. Inorganic pyrophosphatase is a metalloprotease that requires Mg2+ for maximal activity. Although the hydrolysis of inorganic pyrophosphate is specific in the presence of Mg2+, both ADP and ATP can be hydrolyzed if zinc is present.
Form
Type I, lyophilized powder containing 90% buffer salts; Type II, Lyophilized powder containing 85% buffer salts.
Enzyme Commission Number
EC 3.6.1.1
Activity
Type I, > 1,000 units/mg protein (BCA); Type II, > 500 units/mg protein (E1%/280).
CAS No.
9024-82-2
Molecular Weight
71 kDa (homodimer consisting of two equal subunits of molecular weight 32-35 kDa)
Isoelectric point
4.75
Unit Definition
One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25°C.
Storage
−20°C
Synonyms
Pyrophosphate phosphohydrolase; inorganic pyrophosphatase; EC 3.6.1.1; 9024-82-2; iphosphate phosphohydrolase
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Realted Protocols
INORGANIC PYROPHOSPHATASE -Enzymatic Assay Protocol
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