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Native Bovine Carboxypeptidase A

Cat No.
NATE-0150
Description
Carboxypeptidase as isolated from Bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by beta-phenylpropionate and indole acetate.
Abbr
CPA1, Native (Bovine)
Alias
CPA1; CPA
Source
Bovine pancreas
Species
Bovine
Applications
Carboxypeptidase A from bovine pancreas has been used in a study to investigate the expression of a soluble and activatable form of bovine procarboxypeptidase A in Escherichia coli. Carboxypeptidase A from bovine pancreas has also been used in a study to investigate the isolation and partial characterization of precursor forms of ostrich carboxypeptidase. The enzyme from Creative Enzymes has been used as a comparison to study the specificity of Metarhizium anisopliae carboxypeptidase A (MeCPA). MeCPA had been genetically engineered to facilitate the removal of polyhistidine tags from the C-termini of recombinant proteins. It has also been used to de-tyrosinate α-tubulin, in vitro, in order to induce high affinity to ethyl-N-phenylcarbamate (EPC) sepharose.
Product Overview
Protein determined by E1%/278
Form
aqueous suspension.
Enzyme Commission Number
EC 3.4.17.1
Activity
> 50 units/mg protein
CAS No.
11075-17-5
Molecular Weight
mol wt ~35 kDa
Unit Definition
One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25°C.
Storage
2-8°C
Pathway
Pancreatic secretion, organism-specific biosystem; Protein digestion and absorption, organism-specific biosystem; Protein digestion and absorption, conserved biosystem
Function
metallocarboxypeptidase activity; zinc ion binding
Synonyms
EC 3.4.17.1; CPA1; CPA; carboxypeptidase A1; pancreatic procarboxypeptidase A; 11075-17-5; Carboxypolypeptidase; Peptidyl-L-amino-acid hydrolase; carboxypeptidase A; carboxypolypeptidase; pancreatic carboxypeptidase A; tissue carboxypeptidase A
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogEXWM-4061
EC No.EC 3.4.17.1
CAS No.11075-17-5
Source
Realted Protocols
CARBOXYPEPTIDASE P -Enzymatic Assay Protocol
CARBOXYPEPTIDASE G -Enzymatic Assay Protocol
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