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Native Bovine Deoxyribonuclease I RNase-free solution

Cat No.
NATE-0199
Description
Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.
Abbr
DNASE1, Native (Bovine)
Alias
DNASE1; DNase I
Source
Bovine pancreas
Species
Bovine
Applications
Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.
Form
buffered aqueous glycerol solution.
Enzyme Commission Number
EC 3.1.21.1
CAS No.
9003-98-9
Molecular Weight
mol wt 29.1 kDa
Unit Definition
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
Storage
−20°C
Function
actin binding; deoxyribonuclease I activity; endodeoxyribonuclease activity
Synonyms
DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1875
EC No.EC 3.1.21.1
CAS No.9003-98-9
SourceBovine Spleen
CatalogNATE-0198
EC No.EC 3.1.21.1
CAS No.9003-98-9
SourceBovine pancreas
CatalogNATE-0200
EC No.EC 3.1.21.1
CAS No.9003-98-9
SourcePichia pastoris
CatalogNATE-1153
EC No.EC 3.1.21.1
CAS No.
SourceEscherichia coli
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