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Native Brewer's bottom yeast Orotidine-5'-monophosphate pyrophosphorylase

Cat No.
NATE-0498
Description
Orotate phosphoribosyltransferase (OPRTase) or Orotic acid phosphoribosyltransferase is an enzyme involved in pyrimidine biosynthesis. It catalyzes the formation of orotidine 5'-monophosphate (OMP) from orotate and phosphoribosyl pyrophosphate. In yeast and bacteria, orotate phosphoribosyltransferase is an independent enzyme with a unique gene coding for the protein, whereas in mammals and other multicellular organisms, the catalytic function is carried out by a domain of the bifunctional enzyme UMP synthase.
Abbr
OPRTase, Native (Brewer's bottom Yeast)
Alias
OPRTase
Source
Brewer's bottom yeast
Applications
This is the preferred enzyme for assaying orotidine 5′-monophosphate and for the production of OMP analogs from the corresponding orotic acid.
Form
Lyophilized powder containing approx. 50% buffer salts
Enzyme Commission Number
EC 2.4.2.10
Activity
~25 units/mg protein (modified Warburg-Christian)
CAS No.
9030-25-5
Unit Definition
One unit will convert 1.0 μmole of orotidine 5'-monophosphate to orotic acid in one hr at pH 8.0 at 30°C.
Storage
−20°C
Synonyms
orotidylic acid phosphorylase; orotidine-5'-phosphate pyrophosphorylase; OPRTase; orotate phosphoribosyl pyrophosphate transferase; orotic acid phosphoribosyltransferase; orotidine 5'-monophosphate pyrophosphorylase; orotidine monophosphate pyrophosphorylase; orotidine phosphoribosyltransferase; orotidylate phosphoribosyltransferase; orotidylate pyrophosphorylase; orotidylic acid pyrophosphorylase; orotidylic phosphorylase; orotidylic pyrophosphorylase; EC 2.4.2.10; 9030-25-5
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EC No.
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CatalogEXWM-2639
EC No.EC 2.4.2.10
CAS No.9030-25-5
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