Glycerol dehydrogenase is an enzyme in the oxidoreductase family that utilizes the NAD+ to catalyze the oxidation of glycerol to form glycerone (dihydroxyacetone).
GDH, Native (Cellulomonas sp.)
This enzyme is useful for enzymatic determination of glycerol and of triglyceride when coupled with lipoprotein lipase in clinical analysis. Formation of NADH from the reaction of glycerol and NAD+ was catalyzed by the enzyme glycerol dehydrogenase.
Lyophilized powder containing bovine serum albumin
Enzyme Commission Number
50-125 units/mg protein
mol wt ~390 kDa
4.4 ± 0.1
pH 7.5 – 10.5 (25°C, 20hr)
1.1 x 10¯2M (Glycerol), 8.9 x 10¯5M (NAD+)
10 subunits (42 kDa) per mol of enzyme
One unit will oxidize 1.0 μmole of glycerol to dihydroxyacetone per min at 25°C at pH 10.0.
below 55°C (pH 7.5, 15min)
p-Chloromercuribenzoate, o-phenanthroline, monoiodoacetate, heavy metal ions (Co++, Ni++, Cu++, Zn++, Cd++)
EC 220.127.116.11; NAD+-linked glycerol dehydrogenase; glycerol:NAD+ 2-oxidoreductase; GDH; GlDH; GlyDH; 9028-14-2; glycerin dehydrogenase