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Enzymes for Research, Diagnostic and Industrial Use

Native Clostridium sp. Diaphorase

Cat No.
DIA-187
Abbr
Diaphorase (Clostridium sp.)
Alias
Diaphorase
Source
Clostridium sp.
Applications
This enzyme is useful for colorimetric determination of NAD(P)H and many dehydrogenases when coupled with various dyes which act as hydrogen acceptors from NAD(P)H.
Appearance
Yellowish amorphous powder, lyophilized
Form
Freeze dried powder
Enzyme Commission Number
EC 1.6.99.-
Activity
GradeⅢ 30U/mg-solid or more (containing approx. 15% of stabilizers)
Contaminants
Myokinase < 5.0×10⁻¹% NAD(P)H oxidase < 5.0×10⁻¹%
Molecular Weight
24 kDa
pH Stability
pH 7.5 (30°C, 3hr)
Michaelis Constant
2.0×10⁻⁵M (NADH), 6.0×10⁻⁶M (NADPH)
Structure
One mol of FMN per mol of enzyme
Specificity
Either NADH or NADPH can be used as a reductant. The catalytic ratio (NADPH/NADH) is 0.6 in the assay method. Neither oxygen nor cytochrome C can be utilized as a hydrogen acceptor.
Optimum pH
8.5
Optimum temperature
50°C
Thermal stability
below 30°C (pH 7.5, 30min)
Stability
Stable at-20°C for at least one year
Stabilizers
FMN, NAD(P)H
Inhibitors
N-Ethylmaleimide
Synonyms
Diaphorase; EC 1.6.99.-
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