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Native Crotalus adamanteus L-Amino Acid Oxidase

Cat No.
NATE-0366
Description
In enzymology, an L-amino acid oxidase (LAAO) (EC 1.4.3.2) is an enzyme that catalyzes the chemical reaction:an L-amino acid + H2O + O2↔ a 2-oxo acid + NH3 + H2O2. The enzyme was first described in 1944 by A. Zeller and A. Maritz. Not only are LAAOs quite variable in terms of molecular mass, they also vary widely regarding stability. In a similar vein, this enzyme performs in a myriad of biological activities including apoptosis-induction, edema-induction, hemorrhaging, and inhibition or induction of platelet aggregation.
Abbr
LAAO, Native (Crotalus adamanteus)
Alias
LAAO; L-AAO
Source
Crotalus adamanteus
Applications
L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37 oc. L-amino acid oxidase, from Creative Enzymes, has been used in leucine aminopeptidase (LAP) activity assays. The enzyme has been immobilized and used in an enzymatic flow-injection pr ocedure with chemiluminescence detection for on-site determination of L-alanine
Form
Type I, dried venom; Type II, aqueous suspension.
Enzyme Commission Number
EC 1.4.3.2
Activity
Type I, > 0.3 unit/mg solid; Type II, > 3.0 units/mg protein (biuret).
CAS No.
9000-89-9
Unit Definition
One unit will oxidatively deaminate 1.0 μmole of L-phenylalanine per min at pH 6.5 at 37°C. (L-Leucine is deaminated at the same rate at pH 7.8 at 37°C.)
Storage
Type I, −20°C; Type II, 2-8°C.
Buffer
H2O: soluble 1.0 mg/mL, clear (lit.)
Warnings
Dissolves in water at 1 mg/mL concentration to form a clear solution.
Synonyms
L-amino acid oxidase; LAAO; L-AAO; EC 1.4.3.2; 9000-89-9; ophio-amino-acid oxidase; L-amino-acid:oxygen oxidoreductase (deaminating)
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0367
EC No.EC 1.4.3.2
CAS No.9000-89-9
SourceCrotalus atrox (Western Diamondback Rattlesnake)
CatalogNATE-0368
EC No.EC 1.4.3.2
CAS No.9000-89-9
SourceCrotalus durissus venom
CatalogEXWM-1474
EC No.EC 1.4.3.2
CAS No.9000-89-9
Source
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