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Enzymes for Research, Diagnostic and Industrial Use

Native Crotalus adamanteus venom Phosphodiesterase I

Cat No.
NATE-0512
Description
Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+.
Abbr
PDE I, Native (Crotalus adamanteus venom)
Alias
PDE I; 5'-NPDase; 5'-PDase; 5'-PDE; 5'NPDE
Source
Crotalus adamanteus venom
Form
Lyophilized in vials.
Enzyme Commission Number
EC 3.1.4.1
Activity
> 20 units per mg dry weight
CAS No.
9025-82-5
Unit Definition
One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25°C, pH 8.9.
Storage
−20°C
Synonyms
Phosphodiesterase I; EC 3.1.4.1; 5'-exonuclease; 5'-phosphodiesterase; 5'-nucleotide phosphodiesterase; oligonucleate 5'-nucleotidohydrolase; 5' nucleotide phosphodiesterase/alkaline phosphodiesterase I; 5'-NPDase; 5'-PDase; 5'-PDE; 5'NPDE; alkaline phosphodiesterase; nucleotide pyrophosphatase/phosphodiesterase I; orthophosphoric diester phosphohydrolase; PDE I; phosphodiesterase; exonuclease I
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0511
EC No.EC 3.1.4.1
CAS No.9025-82-5
SourceBothrops atrox
CatalogNATE-0513
EC No.EC 3.1.4.1
CAS No.9025-82-5
SourceCrotalus atrox (Western Diamondback Rattlesnake)
CatalogEXWM-3710
EC No.EC 3.1.4.1
CAS No.9025-82-5
Source
Realted Service
Realted Protocols
SPHINGOMYELINASE -Enzymatic Assay Protocol
phosphodieterase -Enzymatic Assay Protocol
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