In enzymology, a L-ascorbate oxidase (EC 188.8.131.52) is an enzyme that catalyzes the chemical reaction:2 L-ascorbate + O2↔ 2 dehydroascorbate + 2 H2O. Thus, the two substRates of this enzyme are L-ascorbate and O2, whereas its two products are dehydroascorbate and H2O. This enzyme belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. This enzyme participates in ascorbate metabolism. It employs one cofactor, copper.
AAO, Native (Cucurbita sp.)
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Creative Enzymes, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis.
Lyophilized powder containing buffers and sucrose as stabilizer.
Enzyme Commission Number
1,000-3,000 units/mg protein
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25°C.
ascorbase; ascorbic acid oxidase; ascorbate oxidase; ascorbic oxidase; ascorbate dehydrogenase; L-ascorbic acid oxidase; AAO; L-ascorbate:O2 oxidoreductase; AA oxidase; EC 184.108.40.206; 9029-44-1; L-ascorbate oxidase