Products

Enzymes for Research, Diagnostic and Industrial Use

Native Diaphorase (NADPH) from Bacillus megaterium

Cat No.
NATE-1154
Description
In enzymology, a NADPH dehydrogenase (EC 1.6.99.1) is an enzyme that catalyzes the chemical reaction: NADPH + H+ + acceptor ↔ NADP+ + reduced acceptor. The 3 substrates of this enzyme are NADPH, H+, and acceptor, whereas its two products are NADP+ and reduced acceptor. This enzyme belongs to the family of oxidoreductases, specifically those acting on NADH or NADPH with other acceptors.
Abbr
Diaphorase (NADPH), Native (Bacillus megaterium)
Source
Bacillus megaterium
Applications
Useful for enzymatic determination of reduced NADP.
Appearance
Yellowish amorphous powder, lyophilized
Form
Freeze dried powder
Enzyme Commission Number
EC 1.6.99.1
Activity
More than 5 U/mg solid
CAS No.
9001-68-7
Contaminants
Myokinase < 0.50%
Molecular Weight
48 kDa (gel filtration)
Isoelectric point
3
pH Stability
6.5‒9.0
Michaelis Constant
NADPH 2.9 × 10-4M
Unit Definition
One unit is defined as the amount of enzyme which oxidizes 1 μmole of NADPH to NADP+ per minute at 30°C under the conditions specified in the assay procedure.
Optimum pH
7.0‒9.0
Activators
FMN, FAD
Thermal stability
Stable at 60°C and below
Storage
At least one year at -20°C
Synonyms
NADPH:acceptor oxidoreductase; NADPH2 diaphorase; NADPH diaphorase; OYE; diaphorase; dihydronicotinamide adenine dinucleotide phosphate dehydrogenase; NADPH-dehydrogenase; NADPH-diaphorase; NADPH2-dehydrogenase; old yellow enzyme; reduced nicotinamide adenine dinucleotide phosphate dehydrogenase; TPNH dehydrogenase; TPNH-diaphorase; triphosphopyridine diaphorase; triphosphopyridine nucleotide diaphorase; NADPH2 dehydrogenase; NADPH: (acceptor) oxidoreductase; NADPH dehydrogenase; EC 1.6.99.1
Download Datasheet:


“Diaphorase (NADPH)” Total Products Page

Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogEXWM-1600
EC No.EC 1.6.99.1
CAS No.9001-68-7
Source
Realted Service
Online Inquiry
Name:
*Phone:
*E-mail Address:
Country:
Company/Institution:
*Products or Services Interested:
Quantity:
Project Description:
*Verification Code:
Please input "enzymes"(case insensitive) as verification code.

Welcome! For price inquiries, please feel free to contact us through the form below through the form on the left side. We will get back to you as soon as possible.

Sitemap  Privacy Policy
Copyright ©2011 - 2017 Creative Enzymes.
Contact Us 45-1 Ramsey Road, Shirley, NY 11967, USA
Email: info@creative-enzymes.com
Tel: 1-631-562-8517 1-631-448-7888
Fax: 1-631-938-8127
Distributors To view the contact information for a specific location, select the desired country: