In enzymology, a ADP-Dependent Hexokinase (EC 188.8.131.52) is an enzyme that catalyzes the chemical reaction: D‒Glucose + ADP → D‒Glucose‒6‒phosphate + AMP.
Hexokinase (ADP-Dependent), Native (Pyrococcus furiosus)
Useful for the enzymatic determination of ADP.
White amorphous powder, lyophilized
Enzyme Commission Number
More than 30 U/mg solid
NADPH oxidase < 0.01%; ATPase < 0.01%
100 kDa (gel filtration) 51 kDa (SDS‒PAGE)
Glucose 0.64 mM (at 37°C) ADP 0.07 mM (at 37°C)
One unit is defined as the amount of enzyme which converts 1 μmole of glucose to D‒Glucose‒6‒phosphate per minute at 37°C under the conditions specified in the assay procedure.
Mg2+, Co2+, Mn2+
Stable at 95°C and below
Storage at －20°C in the presence of a desiccant is recommended.
ADP-dependent glucokinase; ADP-specific glucokinase; ADP:D-glucose 6-phosphotransferase; EC 184.108.40.206