A hexokinase is an enzyme that phosphorylates hexoses (six-carbon sugars), forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, and glucose-6-phosphate the most important product. Hexokinase can transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, which is a specific isoform of hexokinase. While other hexokinases are capable of phosphorylating several hexoses, glucokinase acts with a 50-fold lower substrate affinity and its only hexose substrate is glucose.
Hexokinase, Native (Yeast)
High purity Hexokinase (yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
In 3.2 M ammonium sulphate.
Enzyme Commission Number
98 U/mg protein at pH 7.4 and 25°C.
One Unit of hexokinase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+.
up to 40°C
> 4 years at 4°C
For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.6 containing 5 mM MgCl2 and 0.5 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
hexokinase (phosphorylating); ATP-dependent hexokinase; glucose ATP phosphotransferase; hexokinase; ATP:D-hexose 6-phosphotransferase; EC 126.96.36.199