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Native Microorganism 3α-Hydroxysteroid Dehydrogenase

Cat No.
DIA-270
Description
In enzymology, a 3alpha-hydroxysteroid dehydrogenase (B-specific) (EC 1.1.1.50) is an enzyme that catalyzes the chemical reaction: androsterone + NAD(P)+ ↔ 5alpha-androstane-3,17-dione + NAD(P)H + H+. The 3 substRates of this enzyme are androsterone, NAD+, and NADP+, whereas its 4 products are 5alpha-androstane-3,17-dione, NADH, NADPH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor, more specifically it is part of the group of hydroxysteroid dehydrogenases.
Abbr
3α-Hydroxysteroid Dehydrogenase (Microorganism)
Source
Microorganism
Appearance
White powder, lyophilized
Enzyme Commission Number
EC 1.1.1.50
Activity
> 50U/mg protein
CAS No.
9028-56-2
Molecular Weight
28 kDa (SDS-PAGE)
Isoelectric point
7.5
pH Stability
4.5~10.5
Michaelis Constant
3.0x10-5 M (androsterone); 6.0x10-6 M (NAD+)
Unit Definition
One unit will oxidate one micromole of androsterone per min at pH 8.9 at 25 centigrade.
Optimum pH
10.5
Optimum temperature
60 centigrade
Thermal stability
< 50 centigrade (pH7.2, 20min)
Storage
Storage: Temperature-20 centigrade
Inhibitors
Ag+, Hg2+
Preparation Instructions
The enzyme is reconstituted in 30mM potassium phosphate buffer, pH 7.2 for activity assay.
Synonyms
hydroxyprostaglandin dehydrogenase; 3α-hydroxysteroid oxidoreductase; sterognost 3α; 3α-hydroxysteroid dehydrogenase (B-specific); 3α-hydroxysteroid 3-dehydrogenase (B-specific); 3α-hydroxysteroid:NAD(P)+ 3-oxidoreductase (B-specific); EC 1.1.1.50
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