In enzymology, a pyruvate oxidase (EC 18.104.22.168) is an enzyme that catalyzes the chemical reaction: pyruvate + phosphate + O2 ↔ acetyl phosphate + CO2 + H2O2. The 3 substrates of this enzyme are pyruvate, phosphate, and O2, whereas its 3 products are acetyl phosphate, CO2, and H2O2. This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with oxygen as acceptor. This enzyme participates in pyruvate metabolism. It has 2 cofactors: FAD, and Thiamin diphosphate.
Pyruvate oxidase (Microorganism)
This enzyme is useful for enzymatic determination of pyruvate, GOT, GPT in clinical analysis.
Yellowish amorphous powder, lyophilized
Enzyme Commission Number
GradeⅢ 1.5U/mg-solid or more
ATPase < 5.0×10⁻²% GOT, GPT < 5.0×10⁻²%
approx. 260 kDa
pH 5.7-6.5 (25°C, 20hr)
below 45°C (pH 6.0, 15min)
Stable at-20°C for at least one year
EC 22.214.171.124; pyruvate: oxygen 2-oxidoreductase (phosphorylating); pyruvic oxidase; phosphate-dependent pyruvate oxidase