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Native Nocardia sp. Cholesterol Dehydrogenase

Cat No.
NATE-0892
Description
Cholesterol dehydrogenase is an enzyme that uses nicotinamide adenine dinucleotide/nicotinamide adenine dinucleotide phosphate (NAD(P)) as its cofactor in oxidizing cholesterol to form cholest-4-en-3-one. This enzyme oxidizes the hydroxyl group at the 3 position of the sterol ring to form a ketone.
Abbr
CDH, Native (Nocardia sp.)
Alias
CDH
Source
Nocardia sp.
Applications
Used in the formulation of Cholesterol testing reagents or in biosensor applications.
Appearance
Light yellow to brown powder
Form
Freeze dried powder
Activity
> 5 U/mg
Molecular Weight
37 kDa (SDS-PAGE)
Isoelectric point
4.5
pH Stability
6.5 - 7.5 (37°C, 15 mins)
Unit Definition
One unit of activity is defined as the amount of enzyme that will catalyse the production of 1.0 micromole of NADH per minute at 25°C under standard assay method conditions.
Optimum pH
10
Optimum temperature
30°C
Activators
Triton X-100
Thermal stability
Stable at 35°C and below (pH 7.0, 5 mins)
Inhibitors
Ag+
Synonyms
Cholesterol Dehydrogenase; CDH

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Our Products Cannot Be Used As Medicines Directly For Personal Use.

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