Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP.
Pyruvate Kinase, Native (Thermophillic bacteria)
ATP regeneration in biocatalysis.
Enzyme Commission Number
One unit is defined as the amount of enzyme oxidizing 1 µmol of NADH per one minute from phosphoenolpyruvic acid as a substrate, using ε340=6.22 mM-1cm-1.
100% stability after 1 hour at 85°C
50 mM Tris-HCl (pH 7.5), 50 mM NaCl
Pyruvate kinase; EC 184.108.40.206; 9001-59-6; phosphoenolpyruvate kinase; phosphoenol transphosphorylase; pyruvate kinase (phosphorylating); fluorokinase; fluorokinase (phosphorylating); pyruvic kinase; pyruvate phosphotransferase; ATP:pyruvate 2-O-phosphotransferase