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Native Rabbit Creatine Phosphokinase

Cat No.
NATE-0138
Description
Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP.1 The molecular mass of the protein is found to be approximately 80 kDa Da. It is made up of 2 subunits, each having a molecular weight of 40 kDa ± 2000. The lighter subunit is present in larger amounts.
Abbr
CK, Native (Rabbit)
Alias
CK; CPK; MM-CK; MB-CK; BB-CK
Source
Rabbit muscle
Species
Rabbit
Applications
Molecular Weight: ~81 kDa Creatine Phosphokinase is a dimer composed predominantly of the skeletal muscle derived homodimer (MM). CK also exists as a heterodimer (MB) particularly in the myocardium. CK derived from brain tissue consists mainly of the brain source homodimer (BB). The amino acid sequences of the M chain and B chains are about 80% homologous. From the sequence, the molecular weight of the M chain is 43,112.
Product Overview
Protein determined by biuret.
Form
salt-free, lyophilized powder.
Enzyme Commission Number
EC 2.7.3.2
Activity
> 150 units/mg protein
CAS No.
9001-15-4
Unit Definition
One unit will transfer 1.0 μmole of phosphate from phosphocreatine to ADP per min at pH 7.4 at 30°C.
Optimum pH
pH 8.8-9.0 for the forward reaction and pH 6.0-7.0 for the reverse reaction.
Storage
−20°C
Buffer
0.25 M glycyl-glycine, pH 7.4: soluble 5.0 mg/mL, clear, colorless to slightly yellow
Inhibitors
ADP is a strong inhibitor of the forward reaction competing with ATP. Divalent cations such as Ca2+ (Ki=4.5 mM), Zn2+ and Cu2+ inhibit CK by competing with Mg2+. Other inhibitors include acetate, acetylsalicylic acid, adenosine, p-aminosalicylic acid, AMP, benzoic acid, bicarbonate, bromide, chloride, p-Chloromercuribenzoic acid, ethylene oxide, 2,4-fluorodinitrobenzene, iodide, malonic acid, NAD, nitrate, phosphate, pyrophosphate, salicylic acid, sulfate, sulfite, thyroxine, trichloroacetate, L-triiodothyroxine, L-triiodothyronine, and tripolyphosphate.
Synonyms
EC 2.7.3.2; ATP:creatine phosphotransferase; CK; CPK; MM-CK; MB-CK; BB-CK; creatine phosphokinase; creatine phosphotransferase; phosphocreatine kinase; adenosine triphosphate-creatine transphosphorylase; Mi-CK; CK-BB; CK-MM; CK-MB; CKMiMi; MiMi-CK; 9001-15-4
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Inquiry
Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1869
EC No.EC 2.7.3.2
CAS No.9001-15-4
SourcePorcine Heart
CatalogNATE-1883
EC No.EC 2.7.3.2
CAS No.9001-15-4
SourceHuman Skeletal Muscle
CatalogNATE-1884
EC No.EC 2.7.3.2
CAS No.9001-15-4
SourcePorcine Heart
CatalogNATE-1885
EC No.EC 2.7.3.2
CAS No.9001-15-4
SourceHuman Brain
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Realted Service
Realted Protocols
CREATINE PHOSPHOKINASE -Enzymatic Assay Protocol
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