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Native Rabbit Thrombin

Cat No.
NATE-0700
Description
Thrombin is a serine protease that in humans is encoded by the F2 gene. Prothrombin (coagulation factor II) is proteolytically cleaved to form thrombin in the coagulation cascade, which ultimately results in the reduction of blood loss. Thrombin in turn acts as a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions.
Abbr
Thrombin, Native (Rabbit)
Alias
thrombin
Source
Rabbit plasma
Species
Rabbit
Product Overview
The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1 ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations. Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard.
Enzyme Commission Number
EC 3.4.21.5
Activity
600-2,000 NIH units/mg protein (biuret) or > 2,000 NIH units/mg protein (E1%/280 = 19.5); > 125 NIH units/mg protein (biuret)
CAS No.
9002-04-4
Pathway
Complement and coagulation cascades, organism-specific biosystem (from KEGG) Complement and coagulation cascades, conserved biosystem (from KEGG) Neuroactive ligand-receptor interaction, organism-specific biosystem (from KEGG) Neuroactive ligand-receptor interaction, conserved biosystem (from KEGG) Regulation of actin cytoskeleton, organism-specific biosystem (from KEGG) Regulation of actin cytoskeleton, conserved biosystem (from KEGG)
Synonyms
thrombin; 9002-04-4; EC 3.4.21.5; fibrinogenase; thrombase; thrombofort; topical; thrombin-C; tropostasin; activated blood-coagulation factor II; blood-coagulation factor IIa; factor IIa; E thrombin; β-thrombin; γ-thrombin
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