Aldehyde dehydrogenase from yeast catalyzes the following reaction: RCHO + NAD/NADP+ + H2O → RCOOH + NADH/NADPH+ + H+. The yeast enzyme requires potassium ions and thiols (glutathione, 2-mercaptoethanol, cysteine) for its activity. It is inhibited by traces of heavy metals, particularly copper. Similar enzymes, but with different requirements for their catalytic activities, have been purified from equine liver and from human erythrocytes. The enzyme from yeast has a molecular weight of 200,000.
ALDH, Native (Yeast)
Enzyme Commission Number
The amount of enzyme which will oxidize one micromole of acetaldehyde to acetic acid per minute at 25°C and pH 8.0 in the presence of potassium ions, thiols and §-NAD+.
Aldehyde dehydrogenase (enzyme) solution. Prepare in 0.1 M Tris-HCl, pH 8.0 containing 0.02% bovine serum albumin, to give a final concentration of 0.1-0.2 U/ml. Must be prepared fresh prior to assay.
aldehyde dehydrogenase [NAD(P)+]; Aldehyde: NAD+ (P+) oxi1doreductase; EC 220.127.116.11; Aldehyde Dehydrogenase; ALDH