Phosphotransacetylase converts CoA to acetyl CoA. This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. This enzyme participates in 3 metabolic pathways:taurine and hypotaurine metabolism, pyruvate metabolism, and propanoate metabolism.
PTA, Recombinant (B. subtilis)
High purity recombinant Phosphotransacetylase (B. subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is commonly used, in conjunction with acetate kinase, for the measurement of acetic acid.
In 3.2 M ammonium sulphate.
Enzyme Commission Number
13027 U/mg protein at pH 7.4 and 25°C.
One Unit of phosphotransacetylase is the amount of enzyme required to produce one µmole of acetyl-CoA from coenzyme A per minute measured at 233 nm.
up to 25°C
> 2 years at 4°C
For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
phosphate acetyltransferase; phosphotransacetylase; phosphoacylase; PTA; EC 126.96.36.199; 9029-91-8