Polyphospate Kinase catalyzes the reversible transfer of phosphate between polyphosphate and ATP. The phosphorylation of ADP to ATP by polyphosphate kinase is by a processive mechanism; the phosphorylation occurs without release of the polymer from the enzyme prior to termination of the reaction.
Polyphospate Kinase, Recombinant (Propionibacterium shermanii)
Sterile Filtered White lyophilized (freeze-dried) powder.
Enzyme Commission Number
The amount of Polyphosphate kinase required to convert 1 µmole ADP to ATP per minute at pH 7.5, using polyphosphate as phosphate donor.
Lyophilized Polyphosphate kinase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PPK should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
The protein was lyophilized from 1.15ml PPK solution containing 43.6 U/ml of PPK activity, 0.77 mg/ml total protein, 10mM potassium phosphate pH 6.8 and 25mM sodium polyphosphate.
Polyphosphate kinase; EC 220.127.116.11; Polyphosphoric acid kinase; ATP-polyphosphate phosphotransferase