Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.
PKAC, Recombinant (Human)
>90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
buffered aqueous glycerol solution
>1000 units/mg protein
mol wt 43.5 kDa
One unit will transfer 1 nanomole of phosphate from 32P-ATP to kemptide substrate per minute at pH 7.4 at 30°C (measured by a radioactive filter-binding assay). Km (ATP) = 25 μM at 30°C, pH 7.4. Km (kemptide) = 42 μM (33 μg/ml) at 30°C, pH 7.4.
Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.
Adaptive Immune System, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem
ATP binding; cAMP-dependent protein kinase activity; cAMP-dependent protein kinase activity; nucleotide binding; protein binding; protein kinase binding; protein serine/threonine kinase activity; ubiquitin protein ligase binding
Protein Kinase A catalytic subunit; Protein kinase A; PKA; PKAC; cAMP-dependent protein kinase catalytic subunit; PRKAC