S-adenosylmethionine synthetase (EC 126.96.36.199) (also known as methionine adenosyltransferase (MAT)) is an enzyme that creates S-adenosylmethionine (AdoMet) by reacting methionine (a non-polar amino acid) and ATP (the basic currency of energy). AdoMet is a methyl donor for transmethylation. It gives away its methyl group and is also the propylamino donor in polyamine biosynthesis. S-adenosylmethionine synthetasecan be considered the rate-limiting step of the methionine cycle.
White powder, lyophilized
S-adenosylmethionine (SAM) is a methyl donor and allows DNA methylation. Once DNA is methylated, it switches the genes off and therefore, S-adenosylmethionine can be considered tocontrol gene expression. SAM is also involved in gene transcription, cell proliferation, and production ofsecondary metabolites. Hence SAM synthetase is fast becoming a drug target, inparticular for the following diseases: depression, dementia, vacuolar myelopathy, liver injury, migraine, osteoarthritis, and as a potential cancer chemopreventive agent.
Enzyme Commission Number
About 46kDa (SDS-PAGE detection)
>90% (SDS-PAGE test)
Redissolved in 30% glycerol, 4°C, store at -20°C/-80°C for long-term preservation, Avoid multiple freeze-thaw cycles.
Tris buffer, pH8.0
EC 188.8.131.52; MAT; MATA1; SAMS; SAMS1; Methionine adenosyltransferase 1; S-adenosylmethioninesynthase isoform type-1; AdoMet synthase 1; MAT 1; Methionineadenosyltransferase I/III; MAT-I/III; MAT1A; AMS1