Sucrose phosphorylase (EC. 22.214.171.124) is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. More specifically it has been placed in the retaining glycoside hydrolases family although it catalyzes a transglycosidation rather than hydrolysis. Sucrose phosphorylase catalyzes the conversion of sucrose to D-fructose and α-D-glucose-1-phosphate. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism.
Sucrose phosphorylase, Recombinant (Bifidobacterium adolescentis)
35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol
Enzyme Commission Number
>90% as judged by SDS-PAGE
Sucrose; D- and L-arabinose, D- and L-arabitol and xylitol
This enzyme is shipped at room temperature but should be stored at -20 °C.
Sucrose Phosphorylase; EC 126.96.36.199; sucrose glucosyltransferase; disaccharide glucosyltransferase; Sucrose:orthophosphate α-D-glucosytransferase