In enzymology, a DNA beta-glucosyltransferase is an enzyme that catalyzes the chemical reaction in which a beta-D-glucosyl residue is transferred from UDP-glucose to an hydroxymethylcytosine residue in DNA. It is analogous to the enzyme DNA alpha-glucosyltransferase. This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases.
Useful for the differentiation of hydroxymethylcytosine (HMC) from methylcytosine in DNA, via glucosylating HMC and protecting HMC from endonuclease cleavage.
T4-phage beta-glucosyltransferase, also known as UDP glucose-DNA ?-glucosyltransferase, (Genbank Accession No. NP_049658) amino acids 1-351 (end) with C-terminal His-tag, MW= 41.6 kDa, expressed in E. coli.
aqueous solution, Formulated in 200 mM imidazole and 20% glycerol.
mol wt 41.6 kDa
T4 Beta-glucosyltransferase; DNA beta-glucosyltransferase; T4-HMC-beta-glucosyl transferase; T4-beta-glucosyl transferase; T4 phage beta-glucosyltransferase; UDP glucose-DNA beta-glucosyltransferase; uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase