In enzymology, a dTMP kinase (EC 18.104.22.168) is an enzyme that catalyzes the chemical reaction: ATP + dTMP rightleftharpoons ADP + dTDP. Thus, the two substrates of this enzyme are ATP and dTMP, whereas its two products are ADP and dTDP. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with a phosphate group as acceptor. This enzyme participates in pyrimidine metabolism.
TMPK, Recombinant (Prokaryote)
High purity recombinant Thymidylate kinase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
In 3.2 M ammonium sulphate
Enzyme Commission Number
~ 3.8 U/mg protein at pH 7.6 and 25°C.
~ 25,396 Da
One Unit of thymidylate kinase is defined as the amount of enzyme required to produce one µmole of NAD+ from NADH.
> 2 years at 4°C.
For assay, this enzyme should be diluted in 100 mM TEA buffer, pH 7.6 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use.
dTMP kinase; EC 22.214.171.124; ATP:dTMP phosphotransferase; thymidine monophosphate kinase; thymidylate kinase; thymidylate monophosphate kinase; thymidylic acid kinase; thymidylic kinase; deoxythymidine 5'-monophosphate kinase; TMPK; thymidine 5'-monophosphate kinase