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Deoxyribonuclease I from Bovine, Recombinant
Cat No.
NATE-0200
Description
Deoxyribonuclease I (usually called DNase I), is an endonuclease coded by the human gene DNASE1. DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3', on average producing tetranucleotides. It acts on single-stranded DNA, double-stranded DNA, and chromatin. In addition to its role as a waste-management endonuclease, it has been suggested to be one of the deoxyribonucleases responsible for DNA fragmentation during apoptosis.
Abbr
DNASE1, Recombinant (Bovine pastoris)
Alias
DNASE1; DNase I
Source
Pichia pastoris
Species
Bovine pancreatic
Product Overview
Recombinant Bovine pancreatic Deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases, and proteases. A lyophilized powder containing glycine as a stabilizer.
Form
lyophilized powder
Enzyme Commission Number
EC 3.1.21.1
Activity
> 5000 units per mg protein
CAS No.
9003-98-9
Purity
Chromatographically purified
Unit Definition
One Unit causes an increase in absorbance at 260nm of 0.001 per minute at 25oC when acting upon highly polymerized DNA at pH 5.0.
Storage
Store at 2-8°C
Stabilizers
glycine
Buffer
5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.
Synonyms
DNASE1; deoxyribonuclease I; deoxyribonuclease-1; DNase I; 9003-98-9; EC 3.1.21.1; pancreatic DNase; DNase; thymonuclease, dornase; dornava; dornavac; pancreatic deoxyribonuclease; pancreatic dornase; deoxyribonuclease (pancreatic); pancreatic DNase; DNAase; deoxyribonucleic phosphatase; alkaline deoxyribonuclease; alkaline DNase; endodeoxyribonuclease I; DNA depolymerase; Escherichia coli endonuclease I; deoxyribonuclease A; DNA endonuclease; DNA nuclease
Download
Download Datasheet| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-1875 | Native Bovine Deoxyribonuclease I | EC 3.1.21.1 | 9003-98-9 | Bovine Spleen | Inquiry |
| NATE-0198 | Native Bovine Deoxyribonuclease I | EC 3.1.21.1 | 9003-98-9 | Bovine pancreas | Inquiry |
| NATE-0199 | Native Bovine Deoxyribonuclease I RNase-free solution | EC 3.1.21.1 | 9003-98-9 | Bovine pancreas | Inquiry |
| NATE-1153 | Deoxyribonuclease B, Recombinant | EC 3.1.21.1 | Escherichia coli | Inquiry |