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Sulfite oxidase from H. sapiens, Recombinant
Cat No.
NATE-1229
Description
Sulfite oxidase (EC 1.8.3.1) is an enzyme in the mitochondria of all eukaryotes.[citation needed] It oxidizes sulfite to sulfate and, via cytochrome c, transfers the electrons produced to the electron transport chain, allowing generation of ATP in oxidative phosphorylation. This is the last step in the metabolism of sulfur-containing compounds and the sulfate is excreted. Sulfite oxidase is a metallo-enzyme that utilizes a molybdopterin cofactor and a heme group. It is one of the cytochromes b5 and belongs to the enzyme super-family of molybdenum oxotransferases that also includes DMSO reductase, xanthine oxidase, and nitrite reductase.
Abbr
Sulfite oxidase, Recombinant (H. sapiens)
Source
E. coli
Species
H. sapiens
Form
Supplied in 3.2 M ammonium sulphate
Enzyme Commission Number
EC 1.8.3.1
Activity
0.5 U/mg
CAS No.
9029-38-3
Molecular Weight
approx. 50000 Da
Purity
>95 % as judged by SDS-PAGE
Concentration
1.0 U/ml
Unit Definition
One unit is defined as the amount of enzyme required to oxidize 1.0μmol of sulfite to sulfate, per min, in a coupled assay where the hydrogen peroxide formed in the first reaction is reduced by an NADH-peroxidase in the presence of NADH, at 25 °C and pH 8.5.
Optimum pH
8.5
Optimum temperature
25°C
Storage
Store at 4°C (shipped at room temperature)
Preparation Instructions
Agitate vial sufficiently to fully homogenise enzyme precipitate before use.
Synonyms
sulfite oxidase; EC 1.8.3.1; 9029-38-3
Download
Download Datasheet"Sulfite Oxidase" Total Products Page
| Catalog | Product Name | EC No. | CAS No. | Source | Price |
|---|---|---|---|---|---|
| NATE-0689 | Native Chicken Sulfite Oxidase | EC 1.8.3.1 | 9029-38-3 | Chicken Liver | Inquiry |
| EXWM-1658 | sulfite oxidase | EC 1.8.3.1 | 9029-38-3 | Inquiry |