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α(1-2,3) Mannosidase from Xanthomonas manihotis, Recombinant

Cat No.
NATE-1269
Description
α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins. α-Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.
Abbr
α(1-2,3) Mannosidase, Recombinant (Xanthomonas manihotis)
Source
E. coli
Species
Xanthomonas manihotis
Product Overview
α1-2,3 Mannosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-2 and α1-3 linked D-Mannopyranosyl residues from oligosaccharides.
Form
50 mM NaCl, 20 mM Tris-HCl (pH 7.5 25°C) and 1 mM Na2 EDTA.
Activity
80,000 units/mg
Molecular Weight
90 kDa
Concentration
32,000 units/ml
Unit Definition
One unit is defined as the amount of enzyme required to cleave > 95% of the non-reducing terminal α-D-mannose from 1 nmol Manα1-3Manβ1-4GlcNAc-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 µl.
Storage
4°C
Synonyms
alpha-D-mannosidase; alpha-mannosidase; alpha-D-mannoside mannohydrolase; α-D-Mannosidase; EC 3.2.1.24; 9025-42-7
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