Description
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN).
Abbr
α(1-3,6) Galactosidase, Recombinant (Xanthomonas manihotis)
Species
Xanthomonas manihotis
Product Overview
α1-3,6 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-3 and α1-6 linked D-galactopyranosyl residues from oligosaccharides.
Form
50 mM NaCl, 20 mM Tris-HCl (pH 7.5 25°C) and 1 mM Na2EDTA.
Activity
137,000 units/mg
Molecular Weight
70000 daltons
Purity
> 95% determined by SDS-PAGE
Concentration
4,000 units/ml
Unit Definition
One unit is defined as the amount of enzyme required to cleave > 95% of the terminal, α-D-galactose from 1 nmol Galα1-3Galβ1-4Gal-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 µl.
Storage
Recommended storage temperature is 4°C. Avoid repeated freeze/thaw cycles
Synonyms
Alpha-Galactosidase; Galactosidase; EC 3.2.1.22; GLA; GALA; melibiase; α-D-galactosidase; α-galactosidase A; α-galactoside galactohydrolase
