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α(2→3,6,8,9) Neuraminidase from Arthrobacter ureafaciens, Recombinant

Cat No.
NATE-0758
Description
Neuraminidase enzymes are glycoside hydrolase enzymes (EC 3.2.1.18) that cleave the glycosidic linkages of neuraminic acids. Neuraminidase enzymes are a large family, found in a range of organisms. The best-known neuraminidase is the viral neuraminidase, a drug target for the prevention of the spread of influenza infection. The viral neuraminidases are frequently used as antigenic determinants found on the surface of the Influenza virus. Some variants of the influenza neuraminidase confer more virulence to the virus than others. Other homologs are found in mammalian cells, which have a range of functions.
Abbr
α-Neuraminidase, Recombinant (Arthrobacter ureafaciens)
Alias
sialidase
Source
E. coli
Species
Arthrobacter ureafaciens
Package
Provided with 5×reaction buffer (250 mM sodium phosphate, pH 6.0).
Form
Solution in 20 mM Tris-HCl, pH 7.5, and 20 mM NaCl.
Enzyme Commission Number
EC 3.2.1.18
Activity
> 135 units/mg protein
CAS No.
9001-67-6
Molecular Weight
~88 and ~95 kDa (two active species)
pH Stability
4.5-8.0 (depending upon the substrate)
Unit Definition
One unit will hydrolyze 1 μmole of 4-methylumbelliferyl α-D-N-acetylneuraminide per min at pH 5.0 at 37°C.
Optimum pH
5.0-5.5 (with N-acetylneuraminyllactose) 4.3-4.5 (with colominic acid, poly-N-acetylneuraminic acid)
Stability
Both the enzyme and buffer retain activity for 1 year if stored unopened at 2-8°C. Do Not Freeze. The diluted buffer should be stored at 2-8°C and used within 7 days.
Storage
2-8°C
Preparation Instructions
Expressed in glycosidase-free hosts.
Synonyms
neuraminidase; sialidase; α-neuraminidase; acetylneuraminidase; exo-α-sialidase; EC 3.2.1.18; 9001-67-6
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-1683
EC No.EC 3.2.1.18
CAS No.9001-67-6
Source
CatalogNATE-1716
EC No.EC 3.2.1.18
CAS No.9001-67-6
SourceMicroorganism
CatalogNATE-0756
EC No.EC 3.2.1.18
CAS No.9001-67-6
SourceArthrobacter ureafaciens
CatalogNATE-0757
EC No.EC 3.2.1.18
CAS No.9001-67-6
SourceStreptococcus pneumoniae
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Realted Protocols
neuraminidase -Enzymatic Assay Protocol
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