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β-Amylase from Bacillus cereus, Recombinant

Cat No.
NATE-1052
Description
β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity.
Abbr
β-Amylase, Recombinant (Bacillus cereus)
Source
Bacillus cereus
Applications
Applications established in brewing and distilling industries and in carbohydrate research, particularly in the study of starch and glycogen structures.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.2
Activity
2182 U/mg protein (on soluble Starch) at pH 6.5 and 40°C.
CAS No.
9000-91-3
Molecular Weight
59250
Isoelectric point
~ 6.4
pH Stability
4.0 - 9.0
Unit Definition
One Unit of β-amylase activity is defined as the amount of enzyme required to release one μmole of maltose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) in sodium phosphate buffer (100 mM) pH 6.5.
Optimum pH
6.5
Optimum temperature
40°C
Thermal stability
up to 50°C
Stability
> 2 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
beta-amylase; 4-alpha-D-glucan maltohydrolase; saccharogen amylase; glycogenase; β amylase, β-amylase; 1,4-α-D-glucan maltohydrolase; EC 3.2.1.2; 9000-91-3
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogNATE-0761
EC No.EC 3.2.1.2
CAS No.9000-91-3
SourceBarley
CatalogNATE-0762
EC No.EC 3.2.1.2
CAS No.9000-91-3
SourceSweet potato
CatalogSUG-005
EC No.
CAS No.9000-91-3
Source
CatalogEXWM-3884
ProductNameβ-amylase
EC No.EC 3.2.1.2
CAS No.9000-91-3
Source
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