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Enzymes for Research, Diagnostic and Industrial Use

α-D-Mannosidase from Bacteroides thetaiotaomicron, Recombinant

Cat No.
NATE-1027
Description
α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins. α-Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.
Abbr
α-D-Mannosidase, Recombinant (Bacteroides thetaiotaomicron)
Source
Bacteroides thetaiotaomicron
Applications
For use in research.
Product Overview
High purity α-D-Mannosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.24
Activity
8.2 U/mg protein (on pNP-a-D-mannopyranoside) at pH 6.5 and 40°C
CAS No.
9025-42-7
Molecular Weight
~ 87,000
Isoelectric point
~ 6.53
pH Stability
5.5-8.0
Specificity
Hydrolysis of terminal, non-reducing alpha-D-mannose residues in a-(1,3), a-(1,4) and a-(1,6)-D-mannosides.
Unit Definition
One Unit of a-D-mannosidase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-a-D-mannopyranoside (5 mM) in MES buffer (100 mM) and CaCl2 (2.5 mM) at pH 6.5 at 40°C.
Optimum pH
6.5
Optimum temperature
40°C
Thermal stability
up to 50°C
Stability
> 4 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in MES buffer (100 mM) pH 6.5 containing CaCl2 (2.5 mM) plus 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
alpha-D-mannosidase; alpha-mannosidase; alpha-D-mannoside mannohydrolase; α-D-Mannosidase; EC 3.2.1.24; 9025-42-7
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Realted Protocols
a-mannosidase -Enzymatic Assay Protocol
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