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β-D-Xylosidase from Selenomonas ruminantium, Recombinant

Cat No.
NATE-1134
Description
Releases reducing sugars from birchwood xylan (X0502), also catalyzes the hydrolysis of 4-methylumbelliferyl-β-D-cellobioside and 4-methylumbelliferyl-β-D-glucopyranoside. This enzyme does not possess endo-xylanase, arabinoxylanase or β-glucanase activities. β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability.
Abbr
β-D-Xylosidase, Recombinant (S. ruminantium)
Source
Selenomonas ruminantium
Applications
High purity recombinant exo-1,4-beta-D-Xylosidase (S. ruminantium) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.37
Activity
118 U/mg protein at pH 5.3 and 40°C on p-NP-β-D-xyloside; ~ 145 U/mg protein at pH 5.3 and 40°C on xylobiose.
CAS No.
9025-53-0
Molecular Weight
~ 61,900
Isoelectric point
~ 5.4
Unit Definition
One Unit of β-xylosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (p-NP) per minute from p-nitrophenyl-β-Dxylopyranoside (5 mM) in sodium succinate buffer (50 mM), pH 5.3 at 40°C.
Optimum pH
5.3
Optimum temperature
40°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium succinate buffer (50 mM), pH 5.3 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
β-Xylosidase; EC 3.2.1.37; exo-1,4-β-D-xylosidase; 1,4-β-D-xylan xylohydrolase; β-D-Xylosidase
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