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α-Galactosidase from Penicillium simplicissimum, Recombinant

Cat No.
NATE-1074
Description
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA gene. Two recombinant forms of alpha-galactosidase are called agalsidase alfa (INN) and agalsidase beta (INN).
Abbr
α-Galactosidase, Recombinant (Penicillium simplicissimum)
Alias
GLA
Source
Penicillium simplicissimum
Applications
For use in glycobiology, carbohydrate or biofuels research.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.22
Activity
160 U/mg protein (on p-NP-a-D-galactopyranoside) at pH 3.5 and 40°C.
CAS No.
37288-54-3
Molecular Weight
~ 47,000
Isoelectric point
~ 5.6
pH Stability
3.0 - 9.0
Specificity
Hydrolysis of terminal, non-reducing a-D-galactose residues in a-D-galactosides, including galactose oligosaccharides and galactomannans.
Unit Definition
One Unit of a-galactosidase activity is defined as the amount of enzyme required to release one µmole of of p-nitrophenol (p-NP) per minute from p-nitrophenyl-a-D-galactopyranoside (5 mM) in glycine buffer (100 mM), pH 3.5 at 40°C.
Optimum pH
2.0 - 4.0
Optimum temperature
40 - 50°C
Thermal stability
up to 40°C
Stability
> 2 years at 4°C.
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in glycine buffer (100 mM), pH 3.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
Alpha-Galactosidase; Galactosidase; EC 3.2.1.22; GLA; GALA; melibiase; α-D-galactosidase; α-galactosidase A; α-galactoside galactohydrolase
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a-galactosidase -Enzymatic Assay Protocol
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