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α-Glucosidase from Bacillus stearothermophilus, Recombinant

Cat No.
NATE-1087
Description
Glycoside hydrolases (also called glycosidases or glycosyl hydrolases) assist in the hydrolysis of glycosidic bonds in complex sugars. They are extremely common enzymes with roles in nature including degradation of biomass such as cellulose and hemicellulose, in anti-bacterial defense strategies (e.g., lysozyme), in pathogenesis mechanisms (e.g., viral neuraminidases) and in normal cellular function (e.g., trimming mannosidases involved in N-linked glycoprotein biosynthesis). Together with glycosyltransferases, glycosidases form the major catalytic machinery for the synthesis and breakage of glycosidic bonds.
Abbr
α-Glucosidase, Recombinant (Bacillus stearothermophilus)
Source
Bacillus stearothermophilus
Applications
High purity recombinant alpha-Glucosidase (B. stearothermophilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.20
Activity
138 U/mg protein (on 4-nitrophenyl α-D-glucopyranoside) at pH 6.5 and 40°C ~ 284 U/mg protein (on 4-nitrophenyl α-D-glucopyranoside) at pH 6.5 and 60°C
CAS No.
9001-42-7
Molecular Weight
~ 66,000
Isoelectric point
~ 6.7
pH Stability
5.0 - 9.0
Unit Definition
One Unit of α-D-glucosidase activity is defined as the amount of enzyme required to produce one µmole of p-nitrophenol from 4-nitrophenyl α-D-glucopyranoside per minute at pH 6.5 and 40°C measured at 400 nm.
Optimum pH
6.0 - 6.5
Optimum temperature
60°C
Thermal stability
up to 60°C
Stability
> 4 years at 4°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium phosphate buffer (100 mM), pH 6.5 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
Alpha-glucosidase; EC 3.2.1.20; maltase; glucoinvertase; glucosidosucrase; maltase-glucoamylase; alpha-glucopyranosidase; glucosidoinvertase; alpha-D-glucosidase; alpha-glucoside hydrolase; alpha-1,4-glucosidase; alpha-D-glucoside glucohydrolase; glycosidases; glycosyl hydrolases; α-Glucosidase
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Realted Products
Catalog
ProductName
EC No.
CAS No.
Source
Price
CatalogDIA-194
EC No.EC 3.2.1.20
CAS No.9001-42-7
SourceMicroorganism
CatalogNATE-0041
EC No.EC 3.2.1.20
CAS No.9001-42-7
SourceRice
CatalogNATE-0752
EC No.EC 3.2.1.20
CAS No.9001-42-7
SourceSaccharomyces cerevisiae
CatalogNATE-1083
EC No.EC 3.2.1.20
CAS No.9001-42-7
SourceYeast
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Realted Protocols
MALTASE -Enzymatic Assay Protocol
α-GLUCOSIDASE -Enzymatic Assay Protocol
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