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β-Glucosidase from Phanerochaete chrysosporium, Recombinant

Cat No.
NATE-1090
Description
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one of the cellulases, enzymes involved in the decomposition of cellulose and related polysaccharides; more specifically, an exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of glucose.
Abbr
β-Glucosidase, Recombinant (Phanerochaete chrysosporium)
Source
Phanerochaete chrysosporium
Applications
Highly pure enzyme. Hydrolysis of terminal non-reducing β-D-glucosidic bonds releasing β-D-glucose. CAZy and Biofuels related enzyme.
Form
In 3.2 M ammonium sulphate.
Enzyme Commission Number
EC 3.2.1.21
Activity
103 U/mg protein (on p-NP-β-D-Glucopyranoside) at pH 5.0 and 40°C. ~ 320 U/mg protein (on p-NP-β-D-Glucopyranoside) at pH 5.0 and 60°C.
CAS No.
9001-22-3
Molecular Weight
~ 84,500
Isoelectric point
~ 5.8
pH Stability
3.0 - 9.0
Specificity
Hydrolysis of terminal non-reducing β-D-glucosidic bonds releasing β-D-glucose.
Unit Definition
One Unit of β-glucosidase activity is defined as the amount of enzyme required to release one µmole of of p-nitrophenol (p-NP) per minute from p-nitrophenyl-β-D-glucopyranoside (5 mM) in sodium acetate buffer (100 mM), pH 5.0 at 40°C.
Optimum pH
4.5 - 5.0
Optimum temperature
60 - 70°C
Thermal stability
up to 60°C
Stability
> 4 years at 4°C
Storage
4°C
Preparation Instructions
For assay, this enzyme should be diluted in sodium acetate buffer (100 mM), pH 5.0 containing 1 mg/mL BSA. Swirl to mix the enzyme immediately prior to use.
Synonyms
EC 3.2.1.21; gentiobiase; cellobiase; emulsin; elaterase; aryl-beta-glucosidase; beta-D-glucosidase; beta-glucoside glucohydrolase; arbutinase; amygdalinase; p-nitrophenyl beta-glucosidase; primeverosidase; amygdalase; linamarase; salicilinase; beta-1,6-glucosidase
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Realted Protocols
ß-GLUCOSIDASE -Enzymatic Assay Protocol
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